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Cell Evolution Analysis

The CEA software operates by analyzing sets of cell culture images at distinct observation points T1 and T2. The cell cluster regions denoted as regions of interest are extracted automatically. The areas of the regions are then determined and used to compute the cell evolution rate. CEA can also determine the cell proliferation rate by counting the cells within the ROI

 

CEA Procedure

CEA operation

Modes of Operations

CEA offers two modes of operation for the flexibility and convenience of the user

1. Batch Mode:

This mode provides convenient, user independent operation and batch processing of images without need for user intervention.

2. Calibration Mode:

This mode allows the user to customize CEA behaviour according to his/her special needs and requirements and also enables the user to change the speed and accuracy settings along with the cell counting and accuracy settings along with the cell counting and artifact rejection settings.

CEA Software Interface

CEA provides an intuitive, easy-to-use point-and-click interface GUI that enables users to select images in either batch or calibration modes and store the results in numerical as well as visual formats.

CEA Batch Mode Interface CEA Calibration Mode Interface

Input/Output for batch and calibration modes

Batch Mode Input Output

CEA Performance under varying conditions

 

 

Cell Migration Analysis

The Cell Migration Analysis (CMA) software analyzes the Scratch/Wound video/image sequence frame-by-frame and computes the scratch area dynamically at each time instant

cell migration analysis procedure

Modes of Operation

CMA provides two modes of operation for the convenience of the user.

1. Batch mode operation

For convenient, user-independent processing of multiple sequences of images or videos

2. Single mode

For customization of algorithm parameters for a single image sequence or video based on user preferences

User Interface

CMA provides an easy-to-use, point and click GUI for the convenience of the user.

Input/Output

CMA saves the scratch area in square microns and the percentage of total area for each image or frame as values in an excel sheet. The user can also save the segmented video or image sequences.